The ECNL is divided into 8 regional conferences across the country.  FC Wisconsin competes in the Midwest Conference. The Midwest Conference includes the top 11 female youth soccer clubs in the Midwest:

• Eclipse Select (IL)
• Eclipse Select North (IL)
• Elite Girls Academy (NB)
• FC Wisconsin (WI)
• Kansas City Athletics (MO)
• Michigan Hawks (MI)
• Michigan Hawks Black (MI)
• Minnesota Thunder Academy (MN)
• Vardar SC (MI)
• St. Louis Scott Gallagher - Illinois (IL)
• St. Louis Scott Gallagher - Missouri (MO)

The Midwest Conference is divided into 2 divisions, with every team playing each team in their division twice, and each team in the other division once.